Detection of Ralstonia solanacearum by Loop-Mediated Isothermal Amplification
نویسندگان
چکیده
منابع مشابه
Detection of Ralstonia solanacearum by loop-mediated isothermal amplification.
Ralstonia solanacearum is a pathogenic bacterium that causes wilt in over 200 plant species. Here we report a rapid and sensitive detection of R. solanacearum using an isothermal method for copying DNA known as loop-mediated amplification (LAMP). A set of four primers was designed to replicate the gene coding for the flagellar subunit, fliC, and conditions for detection were optimized to comple...
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[This corrects the article on p. 76 in vol. 8, PMID: 28197157.].
متن کاملLoop-Mediated Isothermal Amplification Method for the Rapid Detection of Ralstonia solanacearum Phylotype I Mulberry Strains in China
Ralstonia solanacearum phylotype I mulberry strains are causative agent of bacterial wilt of mulberry. Current diagnostic methods are not adopted to the mulberry wilt disease. In this study, we developed a rapid method, loop-mediated isothermal amplification (LAMP), to detect R. solanacearum phylotype I mulberry strains. A set of six primers was designed to target the clone MG67 sequence in thi...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2008
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-98-9-1045